<div dir="ltr"><b>Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Translational Medicine and Chemical Biology
</b><p><span>A
postdoctoral position in 3D image analysis of intra-tumor heterogeneity
is immediately available in the Crosetto lab for Quantitative Biology
and Technology (<a href="https://bienkocrosettolabs.org/">https://bienkocrosettolabs.org/</a>)
with the goal of studying phenotypic, genetic, and transcriptional
intra-tumor heterogeneity by high-throughput microscopy imaging of
serial tissue sections from different tumor types and hundreds of
patients. The position is funded through a 33 million SEK research grant
(Integrated Visualization of Intra-Tumor Heterogeneity) recently
awarded to Dr. Crosetto by the Swedish Foundation for Strategic Research
(SSF).<br><br></span><b><span>Research environment<br></span></b><span>The
Crosetto lab is part of the Science for Life Laboratory (SciLifeLab)
situated at the Karolinska Institute Solna campus. SciLifeLab is an
interdisciplinary center for molecular biosciences with focus on health
and environmental research, bringing under the same roof groups from
four universities: Karolinska Institutet, KTH Royal Institute of
Technology, Stockholm University and Uppsala University. The center
features state-of-the-art technology platforms, including
next-generation sequencing, high-throughput histology, super-resolution
microscopy, proteomics, image analysis, and bioinformatics.<br><br></span><span>The
successful candidate will join an interdisciplinary and dynamic team of
international researchers, including clinicians, biologists,
biotechnologists, engineers, computer scientists, and physicists. Our
mission is to transform the way we understand complex biological
phenomena and diseases such as cancer, by integrating next-generation
sequencing technologies, single-molecule microscopy methods, and
advanced computational tools.<br><br></span><b><span>Duties<br></span></b><span>The
main goals of the project are to develop image-based metrics of
phenotypic, genetic, and transcriptional intra-tumor heterogeneity in
various cancer types and clinical samples, and to assess whether these
metrics are predictive of clinical endpoints such as response rate and
overall survival. <br><br></span><span>Specific tasks of the position will include:<br></span><span></span></p><ol><li><span>Develop tools for 2D and 3D automatic segmentation of DAPI-stained nuclei in z-stacked tissue section scans</span></li><li><span>Develop
deep learning approaches (convolutional networks) to automatically
identify different cell types (tumor cells, stroma, blood vessels, etc.)
in the images analyzed, with particular emphasis on identifying
different immune cell types</span></li><li><span>Apply spatial
statistics methods to study the spatial distribution of different cell
types, and define metrics of intra-tumor heterogeneity to be correlated
with clinical endpoints (response rate, survival)</span></li><li><span>Use 3D image data to construct high-resolution maps of the intra-tumor vasculature and model tumor growth</span></li></ol><span>The
successful candidate will be jointly supervised by Dr. N. Crosetto
(supervision on the biological and medical part of the project) as well
as by Dr. K. Smith, Director of the BioImage Informatics national
facility at SciLifeLab Stockholm (supervision on the image analysis
part).<br><br></span><b><span>Entry requirements<br></span></b><span>A person is eligible for a position as postdoctoral research fellow if he or she has obtained a PhD no </span><span>more than seven years before the last date of employment as postdoc.<br><br></span><span>The
successful candidate shall hold a PhD in computer science and/or
physics and/or mathematics and clearly demonstrated prior experience in
image processing, machine learning, and statistical analysis (not
necessarily for biological applications). Proficiency in various
programming languages (C++, Python, Matlab, bash) and knowledge of
software engineering principles (code optimization, parallel computing)
is mandatory. Familiarity with web applications design and visualization
experience is a plus. Prior use of Matlab and/or Python for image
analysis and familiarity with a deep learning framework (Tensorflow,
Caffe, Torch) is highly desirable. Candidates with demonstrated
expertise in biostatistics are particularly encouraged to apply. A
strong motivation to work in an interdisciplinary and collaborative
environment, and a strong sense of mission and self-drive are
indispensable.<br></span><br>Last application date
31.May.2017 11:59 PM CET<br><br>For details and application, please visit<br><a href="https://ki.mynetworkglobal.com/en/what:job/jobID:143481/where:4/">https://ki.mynetworkglobal.com/en/what:job/jobID:143481/where:4/</a></div>